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Pacific Biosciences is looking for a scientist to help improve the biochemistry of its sequencing consumables. The successful candidate will work within a highly multidisciplinary R&D environment to develop novel biochemical assays on both the ensemble and single molecule levels in order to characterize the interactions between fluorescently labeled nucleotide analogs and engineered DNA/RNA polymerases. Other areas of focus will be to improve nucleic acid sample preparation and subsequent sequencing performance as it pertains to polymerase processivity, DNA/RNA integrity, and secondary structure. Ideally, the candidate will have a strong background in biochemistry, chemical biology and/or biophysics with a good working knowledge of chemistry and quantitative data analysis methods. Direct experience with fluorescence spectroscopy, transient state kinetics, and/or single molecule kinetics with nucleic acid-relevant enzymes is a significant plus.
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